pcmv spike (Addgene inc)
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pcmv spike
Pcmv Spike, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plenti+cmv+to+neo+dest/pLenti+CMV+Neo+DEST+(705-1)+(Plasmid+%2317392)/pm41430043-261-11-12
Average 93 stars, based on 60 article reviews
Pcmv Spike, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plenti+cmv+to+neo+dest/pLenti+CMV+Neo+DEST+(705-1)+(Plasmid+%2317392)/pm41430043-261-11-12
Average 93 stars, based on 60 article reviews
pcmv spike - by Bioz Stars,
2026-10
93/100 stars
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Over Expression:Article Title: Oncogene-like induction of cellular invasion from centrosome amplification. Article Snippet: .. For the generation of the inducible PLK4 overexpression system we used the lentiviral vectors pLenti-CMV-TetR-Blast (17492, Addgene) and Article Title: PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage response Article Snippet: .. For inducible PLK4 overexpression in MCF7 cells, the human PLK4 coding sequence was subcloned into Article Title: PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage response. Article Snippet: .. For inducible PLK4 overexpression in MCF7 cells, the human PLK4 coding sequence was subcloned into other:Article Title: The Senescence-Associated-Secretory Phenotype constituting HIF-1α activation is independent of micronuclei induction and associated cGAS-mediated interferon response Article Snippet: In brief, MCF10A cells were cultured in phenol red-free DMEM/F12 (Invitrogen) supplemented with 5% horse serum (Sigma), 20ng/ml epidermal growth factor (EGF; Sigma), 10mg/ml insulin (Invitrogen), 100 mg/ml hydrocortisone (Sigma), 1ng/ml cholera toxin (Sigma) and 100 IU/ml penicillin and 100 μg/ml streptomycin (Invitrogen). Article Title: The Senescence-Associated Secretory Phenotype constitutes HIF-1α activation but is independent of micronuclei-induced cGas/Sting activation Article Snippet: The Senescence-Associated Secretory Phenotype (SASP), characterized by the upregulation of inflammatory cytokines, is triggered during senescence by anti-proliferation stresses, including replicative exhaustion, γ-irradiation, Ras oncogene induction, and centrosome amplification.. The elucidation of common signalling pathway(s) activated in SASP, induced by different antiproliferation stresses, remains an important question.. Indeed, micronuclei activation of the cGAS/Sting pathway, which has been thought to drive SASP(Kwon, Leibowitz, and Lee, 2020), remains controversial(Flynn, Koch, and Mitchison, 2021; Sato and Hayashi, 2024; Takaki et al., 2024). Clone Assay:Article Title: Investigation of the role of tyrosine kinase receptor EPHA3 in colorectal cancer Article Snippet: LS174T ( EPHA3 wild type) and DLD1 (heterozygous EPHA3 T719I and M847K mutations) cells carrying the tet-repressor plasmid (TR1 and TR7, respectively) were generated using the T-rex system (Invitrogen) as described previously . .. To generate EPHA3-inducible clones, DLD1-TR7 and LS174T-TR1 expressing the Tet repressor were transfected with pLenti-CMV/TO-EPHA3 and the corresponding empty vector ( Expressing:Article Title: Investigation of the role of tyrosine kinase receptor EPHA3 in colorectal cancer Article Snippet: LS174T ( EPHA3 wild type) and DLD1 (heterozygous EPHA3 T719I and M847K mutations) cells carrying the tet-repressor plasmid (TR1 and TR7, respectively) were generated using the T-rex system (Invitrogen) as described previously . .. To generate EPHA3-inducible clones, DLD1-TR7 and LS174T-TR1 expressing the Tet repressor were transfected with pLenti-CMV/TO-EPHA3 and the corresponding empty vector ( Transfection:Article Title: Investigation of the role of tyrosine kinase receptor EPHA3 in colorectal cancer Article Snippet: LS174T ( EPHA3 wild type) and DLD1 (heterozygous EPHA3 T719I and M847K mutations) cells carrying the tet-repressor plasmid (TR1 and TR7, respectively) were generated using the T-rex system (Invitrogen) as described previously . .. To generate EPHA3-inducible clones, DLD1-TR7 and LS174T-TR1 expressing the Tet repressor were transfected with pLenti-CMV/TO-EPHA3 and the corresponding empty vector ( Bradford Protein Assay:Article Title: Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption Article Snippet: We are grateful to Judith Simon, Sam Wallis, and all the members of the Godinho lab for comments or discussion of the manuscript; Andrea Lafuente for providing the dot plots used in Figure 2D; Hefin Rhys for helping with ImageStream and Nanosight; and Pedro Monteiro for advice on image analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor Cocktail Cell Signaling #5870 Bradford Protein Assay Bio-Rad #5000006 Recombinant Human TGF-b1 PEPROTECH #100-21 Matrigel Matrix Basement Membrane Life Sciences #354234 Corning Rat Tail High Concentration Life Sciences #354249 DTT VWR Chemicals #M109; CAS: 3483-12-3 Iodoacetamide VWR Chemicals Cat#786-228; CAS: 144-48-9 Trypsin Sigma-Aldrich T6567-1MG L-Arginine Sigma-Aldrich A6969-25G L-Lysine Sigma-Aldrich L8662-25G L-Arginine [U-13C6] Cambridge Isotopes CLM-2265-H-0.5 L-Lysine [4,4,5,5-D4] Cambridge Isotopes DLM-2640-0.5 L-Arginine [U-13C6, U-15N4] Cambridge Isotopes CNLM-539-H-0.5 L-Lysine [U-13C6, U-15N2] Cambridge Isotopes CNLM-291-H-0.5 L-Proline Sigma-Aldrich P0380-100G Critical commercial assays GSH/GSSH-Glo assay Promega #V6611 BCA Protein assay Thermo Fisher Scientific #23225 Magic Red Cathepsin B kit Bio-Rad #ICT937 CellVue Maroon Cell Labeling Kit Invitrogen #88-0870-16 Deposited data SILAC analysis of exosome secretion in response to centrosome amplification This paper PRIDE: PXD020984 accessible via PRIDE partner repository (https://www.ebi. ac.uk/pride/archive/) Experimental models: cell lines PaTu-8988T Prof. Hemant Kocher (QMUL) N/A PaTu-8988S Prof. Yaohe Wang (QMUL) N/A Panc-1 Prof. Hemant Kocher (QMUL) N/A CFPAC-1 Prof. David Pellman (Harvard) N/A Capan-1 Prof. Hemant Kocher (QMUL) N/A HPAF-II Prof. Hemant Kocher (QMUL) N/A MIA-PaCa-2 Prof. Hemant Kocher (QMUL) N/A DEC-hTERT Prof. Hemant Kocher (QMUL) N/A HPDE Prof. Yaohe Wang (QMUL) N/A PS1 Prof. Hemant Kocher (QMUL) N/A HEK293M Prof. David Pellman (Harvard) N/A PaTu-8988S.PLK4 This work N/A HPAF-II.PLK4 This work N/A HPAF-II.PLK4 H2B RFP This work N/A PS1 H2B GFP Angus Cameron (QMUL) N/A Oligonucleotides siSAS6 on-TARGET smart pool Dharmacon #M-004158-02 siNegative Control QIAGEN #1027310 Recombinant DNA pLenti-CMV-TetR-Blast Addgene #17492 Recombinant:Article Title: Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption Article Snippet: We are grateful to Judith Simon, Sam Wallis, and all the members of the Godinho lab for comments or discussion of the manuscript; Andrea Lafuente for providing the dot plots used in Figure 2D; Hefin Rhys for helping with ImageStream and Nanosight; and Pedro Monteiro for advice on image analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor Cocktail Cell Signaling #5870 Bradford Protein Assay Bio-Rad #5000006 Recombinant Human TGF-b1 PEPROTECH #100-21 Matrigel Matrix Basement Membrane Life Sciences #354234 Corning Rat Tail High Concentration Life Sciences #354249 DTT VWR Chemicals #M109; CAS: 3483-12-3 Iodoacetamide VWR Chemicals Cat#786-228; CAS: 144-48-9 Trypsin Sigma-Aldrich T6567-1MG L-Arginine Sigma-Aldrich A6969-25G L-Lysine Sigma-Aldrich L8662-25G L-Arginine [U-13C6] Cambridge Isotopes CLM-2265-H-0.5 L-Lysine [4,4,5,5-D4] Cambridge Isotopes DLM-2640-0.5 L-Arginine [U-13C6, U-15N4] Cambridge Isotopes CNLM-539-H-0.5 L-Lysine [U-13C6, U-15N2] Cambridge Isotopes CNLM-291-H-0.5 L-Proline Sigma-Aldrich P0380-100G Critical commercial assays GSH/GSSH-Glo assay Promega #V6611 BCA Protein assay Thermo Fisher Scientific #23225 Magic Red Cathepsin B kit Bio-Rad #ICT937 CellVue Maroon Cell Labeling Kit Invitrogen #88-0870-16 Deposited data SILAC analysis of exosome secretion in response to centrosome amplification This paper PRIDE: PXD020984 accessible via PRIDE partner repository (https://www.ebi. ac.uk/pride/archive/) Experimental models: cell lines PaTu-8988T Prof. Hemant Kocher (QMUL) N/A PaTu-8988S Prof. Yaohe Wang (QMUL) N/A Panc-1 Prof. Hemant Kocher (QMUL) N/A CFPAC-1 Prof. David Pellman (Harvard) N/A Capan-1 Prof. Hemant Kocher (QMUL) N/A HPAF-II Prof. Hemant Kocher (QMUL) N/A MIA-PaCa-2 Prof. Hemant Kocher (QMUL) N/A DEC-hTERT Prof. Hemant Kocher (QMUL) N/A HPDE Prof. Yaohe Wang (QMUL) N/A PS1 Prof. Hemant Kocher (QMUL) N/A HEK293M Prof. David Pellman (Harvard) N/A PaTu-8988S.PLK4 This work N/A HPAF-II.PLK4 This work N/A HPAF-II.PLK4 H2B RFP This work N/A PS1 H2B GFP Angus Cameron (QMUL) N/A Oligonucleotides siSAS6 on-TARGET smart pool Dharmacon #M-004158-02 siNegative Control QIAGEN #1027310 Recombinant DNA pLenti-CMV-TetR-Blast Addgene #17492 Membrane:Article Title: Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption Article Snippet: We are grateful to Judith Simon, Sam Wallis, and all the members of the Godinho lab for comments or discussion of the manuscript; Andrea Lafuente for providing the dot plots used in Figure 2D; Hefin Rhys for helping with ImageStream and Nanosight; and Pedro Monteiro for advice on image analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor Cocktail Cell Signaling #5870 Bradford Protein Assay Bio-Rad #5000006 Recombinant Human TGF-b1 PEPROTECH #100-21 Matrigel Matrix Basement Membrane Life Sciences #354234 Corning Rat Tail High Concentration Life Sciences #354249 DTT VWR Chemicals #M109; CAS: 3483-12-3 Iodoacetamide VWR Chemicals Cat#786-228; CAS: 144-48-9 Trypsin Sigma-Aldrich T6567-1MG L-Arginine Sigma-Aldrich A6969-25G L-Lysine Sigma-Aldrich L8662-25G L-Arginine [U-13C6] Cambridge Isotopes CLM-2265-H-0.5 L-Lysine [4,4,5,5-D4] Cambridge Isotopes DLM-2640-0.5 L-Arginine [U-13C6, U-15N4] Cambridge Isotopes CNLM-539-H-0.5 L-Lysine [U-13C6, U-15N2] Cambridge Isotopes CNLM-291-H-0.5 L-Proline Sigma-Aldrich P0380-100G Critical commercial assays GSH/GSSH-Glo assay Promega #V6611 BCA Protein assay Thermo Fisher Scientific #23225 Magic Red Cathepsin B kit Bio-Rad #ICT937 CellVue Maroon Cell Labeling Kit Invitrogen #88-0870-16 Deposited data SILAC analysis of exosome secretion in response to centrosome amplification This paper PRIDE: PXD020984 accessible via PRIDE partner repository (https://www.ebi. ac.uk/pride/archive/) Experimental models: cell lines PaTu-8988T Prof. Hemant Kocher (QMUL) N/A PaTu-8988S Prof. Yaohe Wang (QMUL) N/A Panc-1 Prof. Hemant Kocher (QMUL) N/A CFPAC-1 Prof. David Pellman (Harvard) N/A Capan-1 Prof. Hemant Kocher (QMUL) N/A HPAF-II Prof. Hemant Kocher (QMUL) N/A MIA-PaCa-2 Prof. Hemant Kocher (QMUL) N/A DEC-hTERT Prof. Hemant Kocher (QMUL) N/A HPDE Prof. Yaohe Wang (QMUL) N/A PS1 Prof. Hemant Kocher (QMUL) N/A HEK293M Prof. David Pellman (Harvard) N/A PaTu-8988S.PLK4 This work N/A HPAF-II.PLK4 This work N/A HPAF-II.PLK4 H2B RFP This work N/A PS1 H2B GFP Angus Cameron (QMUL) N/A Oligonucleotides siSAS6 on-TARGET smart pool Dharmacon #M-004158-02 siNegative Control QIAGEN #1027310 Recombinant DNA pLenti-CMV-TetR-Blast Addgene #17492 Concentration Assay:Article Title: Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption Article Snippet: We are grateful to Judith Simon, Sam Wallis, and all the members of the Godinho lab for comments or discussion of the manuscript; Andrea Lafuente for providing the dot plots used in Figure 2D; Hefin Rhys for helping with ImageStream and Nanosight; and Pedro Monteiro for advice on image analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor Cocktail Cell Signaling #5870 Bradford Protein Assay Bio-Rad #5000006 Recombinant Human TGF-b1 PEPROTECH #100-21 Matrigel Matrix Basement Membrane Life Sciences #354234 Corning Rat Tail High Concentration Life Sciences #354249 DTT VWR Chemicals #M109; CAS: 3483-12-3 Iodoacetamide VWR Chemicals Cat#786-228; CAS: 144-48-9 Trypsin Sigma-Aldrich T6567-1MG L-Arginine Sigma-Aldrich A6969-25G L-Lysine Sigma-Aldrich L8662-25G L-Arginine [U-13C6] Cambridge Isotopes CLM-2265-H-0.5 L-Lysine [4,4,5,5-D4] Cambridge Isotopes DLM-2640-0.5 L-Arginine [U-13C6, U-15N4] Cambridge Isotopes CNLM-539-H-0.5 L-Lysine [U-13C6, U-15N2] Cambridge Isotopes CNLM-291-H-0.5 L-Proline Sigma-Aldrich P0380-100G Critical commercial assays GSH/GSSH-Glo assay Promega #V6611 BCA Protein assay Thermo Fisher Scientific #23225 Magic Red Cathepsin B kit Bio-Rad #ICT937 CellVue Maroon Cell Labeling Kit Invitrogen #88-0870-16 Deposited data SILAC analysis of exosome secretion in response to centrosome amplification This paper PRIDE: PXD020984 accessible via PRIDE partner repository (https://www.ebi. ac.uk/pride/archive/) Experimental models: cell lines PaTu-8988T Prof. Hemant Kocher (QMUL) N/A PaTu-8988S Prof. Yaohe Wang (QMUL) N/A Panc-1 Prof. Hemant Kocher (QMUL) N/A CFPAC-1 Prof. David Pellman (Harvard) N/A Capan-1 Prof. Hemant Kocher (QMUL) N/A HPAF-II Prof. Hemant Kocher (QMUL) N/A MIA-PaCa-2 Prof. Hemant Kocher (QMUL) N/A DEC-hTERT Prof. Hemant Kocher (QMUL) N/A HPDE Prof. Yaohe Wang (QMUL) N/A PS1 Prof. Hemant Kocher (QMUL) N/A HEK293M Prof. David Pellman (Harvard) N/A PaTu-8988S.PLK4 This work N/A HPAF-II.PLK4 This work N/A HPAF-II.PLK4 H2B RFP This work N/A PS1 H2B GFP Angus Cameron (QMUL) N/A Oligonucleotides siSAS6 on-TARGET smart pool Dharmacon #M-004158-02 siNegative Control QIAGEN #1027310 Recombinant DNA pLenti-CMV-TetR-Blast Addgene #17492 Bicinchoninic Acid Protein Assay:Article Title: Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption Article Snippet: We are grateful to Judith Simon, Sam Wallis, and all the members of the Godinho lab for comments or discussion of the manuscript; Andrea Lafuente for providing the dot plots used in Figure 2D; Hefin Rhys for helping with ImageStream and Nanosight; and Pedro Monteiro for advice on image analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor Cocktail Cell Signaling #5870 Bradford Protein Assay Bio-Rad #5000006 Recombinant Human TGF-b1 PEPROTECH #100-21 Matrigel Matrix Basement Membrane Life Sciences #354234 Corning Rat Tail High Concentration Life Sciences #354249 DTT VWR Chemicals #M109; CAS: 3483-12-3 Iodoacetamide VWR Chemicals Cat#786-228; CAS: 144-48-9 Trypsin Sigma-Aldrich T6567-1MG L-Arginine Sigma-Aldrich A6969-25G L-Lysine Sigma-Aldrich L8662-25G L-Arginine [U-13C6] Cambridge Isotopes CLM-2265-H-0.5 L-Lysine [4,4,5,5-D4] Cambridge Isotopes DLM-2640-0.5 L-Arginine [U-13C6, U-15N4] Cambridge Isotopes CNLM-539-H-0.5 L-Lysine [U-13C6, U-15N2] Cambridge Isotopes CNLM-291-H-0.5 L-Proline Sigma-Aldrich P0380-100G Critical commercial assays GSH/GSSH-Glo assay Promega #V6611 BCA Protein assay Thermo Fisher Scientific #23225 Magic Red Cathepsin B kit Bio-Rad #ICT937 CellVue Maroon Cell Labeling Kit Invitrogen #88-0870-16 Deposited data SILAC analysis of exosome secretion in response to centrosome amplification This paper PRIDE: PXD020984 accessible via PRIDE partner repository (https://www.ebi. ac.uk/pride/archive/) Experimental models: cell lines PaTu-8988T Prof. Hemant Kocher (QMUL) N/A PaTu-8988S Prof. Yaohe Wang (QMUL) N/A Panc-1 Prof. Hemant Kocher (QMUL) N/A CFPAC-1 Prof. David Pellman (Harvard) N/A Capan-1 Prof. Hemant Kocher (QMUL) N/A HPAF-II Prof. Hemant Kocher (QMUL) N/A MIA-PaCa-2 Prof. Hemant Kocher (QMUL) N/A DEC-hTERT Prof. Hemant Kocher (QMUL) N/A HPDE Prof. Yaohe Wang (QMUL) N/A PS1 Prof. Hemant Kocher (QMUL) N/A HEK293M Prof. David Pellman (Harvard) N/A PaTu-8988S.PLK4 This work N/A HPAF-II.PLK4 This work N/A HPAF-II.PLK4 H2B RFP This work N/A PS1 H2B GFP Angus Cameron (QMUL) N/A Oligonucleotides siSAS6 on-TARGET smart pool Dharmacon #M-004158-02 siNegative Control QIAGEN #1027310 Recombinant DNA pLenti-CMV-TetR-Blast Addgene #17492 Labeling:Article Title: Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption Article Snippet: We are grateful to Judith Simon, Sam Wallis, and all the members of the Godinho lab for comments or discussion of the manuscript; Andrea Lafuente for providing the dot plots used in Figure 2D; Hefin Rhys for helping with ImageStream and Nanosight; and Pedro Monteiro for advice on image analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor Cocktail Cell Signaling #5870 Bradford Protein Assay Bio-Rad #5000006 Recombinant Human TGF-b1 PEPROTECH #100-21 Matrigel Matrix Basement Membrane Life Sciences #354234 Corning Rat Tail High Concentration Life Sciences #354249 DTT VWR Chemicals #M109; CAS: 3483-12-3 Iodoacetamide VWR Chemicals Cat#786-228; CAS: 144-48-9 Trypsin Sigma-Aldrich T6567-1MG L-Arginine Sigma-Aldrich A6969-25G L-Lysine Sigma-Aldrich L8662-25G L-Arginine [U-13C6] Cambridge Isotopes CLM-2265-H-0.5 L-Lysine [4,4,5,5-D4] Cambridge Isotopes DLM-2640-0.5 L-Arginine [U-13C6, U-15N4] Cambridge Isotopes CNLM-539-H-0.5 L-Lysine [U-13C6, U-15N2] Cambridge Isotopes CNLM-291-H-0.5 L-Proline Sigma-Aldrich P0380-100G Critical commercial assays GSH/GSSH-Glo assay Promega #V6611 BCA Protein assay Thermo Fisher Scientific #23225 Magic Red Cathepsin B kit Bio-Rad #ICT937 CellVue Maroon Cell Labeling Kit Invitrogen #88-0870-16 Deposited data SILAC analysis of exosome secretion in response to centrosome amplification This paper PRIDE: PXD020984 accessible via PRIDE partner repository (https://www.ebi. ac.uk/pride/archive/) Experimental models: cell lines PaTu-8988T Prof. Hemant Kocher (QMUL) N/A PaTu-8988S Prof. Yaohe Wang (QMUL) N/A Panc-1 Prof. Hemant Kocher (QMUL) N/A CFPAC-1 Prof. David Pellman (Harvard) N/A Capan-1 Prof. Hemant Kocher (QMUL) N/A HPAF-II Prof. Hemant Kocher (QMUL) N/A MIA-PaCa-2 Prof. Hemant Kocher (QMUL) N/A DEC-hTERT Prof. Hemant Kocher (QMUL) N/A HPDE Prof. Yaohe Wang (QMUL) N/A PS1 Prof. Hemant Kocher (QMUL) N/A HEK293M Prof. David Pellman (Harvard) N/A PaTu-8988S.PLK4 This work N/A HPAF-II.PLK4 This work N/A HPAF-II.PLK4 H2B RFP This work N/A PS1 H2B GFP Angus Cameron (QMUL) N/A Oligonucleotides siSAS6 on-TARGET smart pool Dharmacon #M-004158-02 siNegative Control QIAGEN #1027310 Recombinant DNA pLenti-CMV-TetR-Blast Addgene #17492 Multiplex sample analysis:Article Title: Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption Article Snippet: We are grateful to Judith Simon, Sam Wallis, and all the members of the Godinho lab for comments or discussion of the manuscript; Andrea Lafuente for providing the dot plots used in Figure 2D; Hefin Rhys for helping with ImageStream and Nanosight; and Pedro Monteiro for advice on image analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor Cocktail Cell Signaling #5870 Bradford Protein Assay Bio-Rad #5000006 Recombinant Human TGF-b1 PEPROTECH #100-21 Matrigel Matrix Basement Membrane Life Sciences #354234 Corning Rat Tail High Concentration Life Sciences #354249 DTT VWR Chemicals #M109; CAS: 3483-12-3 Iodoacetamide VWR Chemicals Cat#786-228; CAS: 144-48-9 Trypsin Sigma-Aldrich T6567-1MG L-Arginine Sigma-Aldrich A6969-25G L-Lysine Sigma-Aldrich L8662-25G L-Arginine [U-13C6] Cambridge Isotopes CLM-2265-H-0.5 L-Lysine [4,4,5,5-D4] Cambridge Isotopes DLM-2640-0.5 L-Arginine [U-13C6, U-15N4] Cambridge Isotopes CNLM-539-H-0.5 L-Lysine [U-13C6, U-15N2] Cambridge Isotopes CNLM-291-H-0.5 L-Proline Sigma-Aldrich P0380-100G Critical commercial assays GSH/GSSH-Glo assay Promega #V6611 BCA Protein assay Thermo Fisher Scientific #23225 Magic Red Cathepsin B kit Bio-Rad #ICT937 CellVue Maroon Cell Labeling Kit Invitrogen #88-0870-16 Deposited data SILAC analysis of exosome secretion in response to centrosome amplification This paper PRIDE: PXD020984 accessible via PRIDE partner repository (https://www.ebi. ac.uk/pride/archive/) Experimental models: cell lines PaTu-8988T Prof. Hemant Kocher (QMUL) N/A PaTu-8988S Prof. Yaohe Wang (QMUL) N/A Panc-1 Prof. Hemant Kocher (QMUL) N/A CFPAC-1 Prof. David Pellman (Harvard) N/A Capan-1 Prof. Hemant Kocher (QMUL) N/A HPAF-II Prof. Hemant Kocher (QMUL) N/A MIA-PaCa-2 Prof. Hemant Kocher (QMUL) N/A DEC-hTERT Prof. Hemant Kocher (QMUL) N/A HPDE Prof. Yaohe Wang (QMUL) N/A PS1 Prof. Hemant Kocher (QMUL) N/A HEK293M Prof. David Pellman (Harvard) N/A PaTu-8988S.PLK4 This work N/A HPAF-II.PLK4 This work N/A HPAF-II.PLK4 H2B RFP This work N/A PS1 H2B GFP Angus Cameron (QMUL) N/A Oligonucleotides siSAS6 on-TARGET smart pool Dharmacon #M-004158-02 siNegative Control QIAGEN #1027310 Recombinant DNA pLenti-CMV-TetR-Blast Addgene #17492 Amplification:Article Title: Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption Article Snippet: We are grateful to Judith Simon, Sam Wallis, and all the members of the Godinho lab for comments or discussion of the manuscript; Andrea Lafuente for providing the dot plots used in Figure 2D; Hefin Rhys for helping with ImageStream and Nanosight; and Pedro Monteiro for advice on image analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor Cocktail Cell Signaling #5870 Bradford Protein Assay Bio-Rad #5000006 Recombinant Human TGF-b1 PEPROTECH #100-21 Matrigel Matrix Basement Membrane Life Sciences #354234 Corning Rat Tail High Concentration Life Sciences #354249 DTT VWR Chemicals #M109; CAS: 3483-12-3 Iodoacetamide VWR Chemicals Cat#786-228; CAS: 144-48-9 Trypsin Sigma-Aldrich T6567-1MG L-Arginine Sigma-Aldrich A6969-25G L-Lysine Sigma-Aldrich L8662-25G L-Arginine [U-13C6] Cambridge Isotopes CLM-2265-H-0.5 L-Lysine [4,4,5,5-D4] Cambridge Isotopes DLM-2640-0.5 L-Arginine [U-13C6, U-15N4] Cambridge Isotopes CNLM-539-H-0.5 L-Lysine [U-13C6, U-15N2] Cambridge Isotopes CNLM-291-H-0.5 L-Proline Sigma-Aldrich P0380-100G Critical commercial assays GSH/GSSH-Glo assay Promega #V6611 BCA Protein assay Thermo Fisher Scientific #23225 Magic Red Cathepsin B kit Bio-Rad #ICT937 CellVue Maroon Cell Labeling Kit Invitrogen #88-0870-16 Deposited data SILAC analysis of exosome secretion in response to centrosome amplification This paper PRIDE: PXD020984 accessible via PRIDE partner repository (https://www.ebi. ac.uk/pride/archive/) Experimental models: cell lines PaTu-8988T Prof. Hemant Kocher (QMUL) N/A PaTu-8988S Prof. Yaohe Wang (QMUL) N/A Panc-1 Prof. Hemant Kocher (QMUL) N/A CFPAC-1 Prof. David Pellman (Harvard) N/A Capan-1 Prof. Hemant Kocher (QMUL) N/A HPAF-II Prof. Hemant Kocher (QMUL) N/A MIA-PaCa-2 Prof. Hemant Kocher (QMUL) N/A DEC-hTERT Prof. Hemant Kocher (QMUL) N/A HPDE Prof. Yaohe Wang (QMUL) N/A PS1 Prof. Hemant Kocher (QMUL) N/A HEK293M Prof. David Pellman (Harvard) N/A PaTu-8988S.PLK4 This work N/A HPAF-II.PLK4 This work N/A HPAF-II.PLK4 H2B RFP This work N/A PS1 H2B GFP Angus Cameron (QMUL) N/A Oligonucleotides siSAS6 on-TARGET smart pool Dharmacon #M-004158-02 siNegative Control QIAGEN #1027310 Recombinant DNA pLenti-CMV-TetR-Blast Addgene #17492 Control:Article Title: Centrosome amplification mediates small extracellular vesicle secretion via lysosome disruption Article Snippet: We are grateful to Judith Simon, Sam Wallis, and all the members of the Godinho lab for comments or discussion of the manuscript; Andrea Lafuente for providing the dot plots used in Figure 2D; Hefin Rhys for helping with ImageStream and Nanosight; and Pedro Monteiro for advice on image analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor Cocktail Cell Signaling #5870 Bradford Protein Assay Bio-Rad #5000006 Recombinant Human TGF-b1 PEPROTECH #100-21 Matrigel Matrix Basement Membrane Life Sciences #354234 Corning Rat Tail High Concentration Life Sciences #354249 DTT VWR Chemicals #M109; CAS: 3483-12-3 Iodoacetamide VWR Chemicals Cat#786-228; CAS: 144-48-9 Trypsin Sigma-Aldrich T6567-1MG L-Arginine Sigma-Aldrich A6969-25G L-Lysine Sigma-Aldrich L8662-25G L-Arginine [U-13C6] Cambridge Isotopes CLM-2265-H-0.5 L-Lysine [4,4,5,5-D4] Cambridge Isotopes DLM-2640-0.5 L-Arginine [U-13C6, U-15N4] Cambridge Isotopes CNLM-539-H-0.5 L-Lysine [U-13C6, U-15N2] Cambridge Isotopes CNLM-291-H-0.5 L-Proline Sigma-Aldrich P0380-100G Critical commercial assays GSH/GSSH-Glo assay Promega #V6611 BCA Protein assay Thermo Fisher Scientific #23225 Magic Red Cathepsin B kit Bio-Rad #ICT937 CellVue Maroon Cell Labeling Kit Invitrogen #88-0870-16 Deposited data SILAC analysis of exosome secretion in response to centrosome amplification This paper PRIDE: PXD020984 accessible via PRIDE partner repository (https://www.ebi. ac.uk/pride/archive/) Experimental models: cell lines PaTu-8988T Prof. Hemant Kocher (QMUL) N/A PaTu-8988S Prof. Yaohe Wang (QMUL) N/A Panc-1 Prof. Hemant Kocher (QMUL) N/A CFPAC-1 Prof. David Pellman (Harvard) N/A Capan-1 Prof. Hemant Kocher (QMUL) N/A HPAF-II Prof. Hemant Kocher (QMUL) N/A MIA-PaCa-2 Prof. Hemant Kocher (QMUL) N/A DEC-hTERT Prof. Hemant Kocher (QMUL) N/A HPDE Prof. Yaohe Wang (QMUL) N/A PS1 Prof. Hemant Kocher (QMUL) N/A HEK293M Prof. David Pellman (Harvard) N/A PaTu-8988S.PLK4 This work N/A HPAF-II.PLK4 This work N/A HPAF-II.PLK4 H2B RFP This work N/A PS1 H2B GFP Angus Cameron (QMUL) N/A Oligonucleotides siSAS6 on-TARGET smart pool Dharmacon #M-004158-02 siNegative Control QIAGEN #1027310 Recombinant DNA pLenti-CMV-TetR-Blast Addgene #17492 Sequencing:Article Title: PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage response Article Snippet: .. For inducible PLK4 overexpression in MCF7 cells, the human PLK4 coding sequence was subcloned into Article Title: PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage response. Article Snippet: .. For inducible PLK4 overexpression in MCF7 cells, the human PLK4 coding sequence was subcloned into |
