pcmv spike  (Addgene inc)

Journal: bioRxiv

Article Title: The functional organisation of retrosplenial feedback to V1

doi: 10.1101/2025.09.25.678583

Figure Lengend Snippet: (A) Schematic of injection for labelling of RSC→V1 axons and two-photon recording. (B) Example field of view showing RSC axons in layer 1 of V1 labelled with jGCaMP8s. (C) Example spatial responses (average traces plotted over heatmap) for three visually responsive boutons. (D) 2d Gaussian fits of the spatial receptive fields of boutons shown in C. (E) Distribution of spatial receptive field size (sigma from fit) in azimuth and elevation. (F) Distribution of fitted preferred position of each RSC→V1 bouton relative to the preferred position of other boutons in the same field of view. n = 1950 boutons collected from 8 mice in 12 experiments.

Article Snippet: After the brain was exposed, an AAV was injected at a depth of 400μm to drive expression of jGCaMP8s (pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3, Addgene plasmid #172921, 5 × 10 12 GC per millilitre titre, volume 100 nL).

Techniques: Injection

Journal: bioRxiv

Article Title: The functional organisation of retrosplenial feedback to V1

doi: 10.1101/2025.09.25.678583

Figure Lengend Snippet: (A) Schematic of injection for labelling of V1 somas with jRGECO1a and RSC→V1 axons with jGCaMP8s and two-photon recording. (B) Example field of view showing RSC axons in layer 1 of V1 labelled with jGCaMP8s and layer 2/3 somas labelled with jRGECO1a. (C) Example spatial responses (average traces plotted over heatmap) for three visually responsive boutons (left) and 3 retinotopically aligned V1 somas (right). (D) Heat plot showing distribution of difference in tuning of all RSC→V1 boutons relative to local V1 neurons in azimuth and elevation. (E) Responses of each RSC→V1 bouton to expanding versus contracting stimulus. (F) Data from D divided into binocular and monocular areas of the V1 retinotopic map showing nasal offset of RSC→V1 bouton positional tuning relative to V1 tuning in monocular area. (G) Distribution of ΔV1-RSC bouton positional preference showing offset is specific to monocular visual space. For V1 soma recordings, n = 827 neurons from 9 experiments in 6 mice. For RSC→V1 bouton recordings, n = 1467 boutons from 9 experiments in the same 6 mice used for soma recordings.

Article Snippet: After the brain was exposed, an AAV was injected at a depth of 400μm to drive expression of jGCaMP8s (pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3, Addgene plasmid #172921, 5 × 10 12 GC per millilitre titre, volume 100 nL).

Techniques: Injection